Search resultsEffect of lifestyle factors on plasma total homocysteine concentrations in relation to MTHFR(C677T) genotype. Inter99 (7)
Title
Effect of lifestyle factors on plasma total homocysteine concentrations in relation to MTHFR(C677T) genotype. Inter99 (7)
Publication type
Journal Article
Year of Publication
2004
Authors
Journal
European Journal of Clinical Nutrition
Volume
58
Issue
8
Pagination
1142 - 1150
Date published
2004
ISBN
09543007 (ISSN)
Keywords
Adult, age, alcohol consumption, Alcohol Drinking, article, Cardiovascular Diseases, cardiovascular risk, coffee, controlled study, Cross-Sectional Studies, Denmark, eating habit, Exercise, Female, fluorescence polarization immunoassay, Food Habits, gene interaction, Gene-lifestyle interaction, genotype, Health Surveys, homocysteine, human, Humans, Life Style, lifestyle, male, medical examination, Methylenetetrahydrofolate Reductase (NADPH2), Middle Aged, MTHFR, physical activity, polymerase chain reaction, Polymorphism, Restriction Fragment Length, population research, Population study, protein blood level, questionnaire, Questionnaires, randomization, restriction fragment length polymorphism, Risk Factors, sex difference, smoking, smoking habit, statistical analysis, tea
Abstract
Objective: To examine the associations between various lifestyle factors - smoking habits, physical activity, dietary habits, coffee, tea, and alcohol consumption - and homocysteine (tHcy) in relation to MTHFR(C677T) genotype. Design: Cross-sectional population-based study. Setting: Residents of Copenhagen County, Denmark. Subjects: A random sample of 6457 men and women aged 30-60 years drawn from the Civil Registration System and invited to a health examination in 1999-2001. A total of 2788 participants were included in the statistical analysis. Main outcome measures: tHcy was measured using a Fluorescent Polarization Immuno Assay. MTHFR-genotype was determined by PCR and RFLP analysis. Information about lifestyle factors was obtained from a self-administered questionnaire. Results: Daily smoking, less healthy dietary habits, and coffee drinking were associated with elevated tHcy concentrations independent of other determinants. Wine consumption was related to tHcy in a J-shaped manner, whereas beer consumption was negatively associated with tHcy after multiple adjustments. Interaction was observed between smoking status and MTHFR-genotype, smoking status and sex, and beer consumption and age. The effect of smoking was more pronounced in persons with the TT genotype and in women. The effect of beer consumption was more pronounced at younger than at older ages. Conclusions: Smoking status, dietary habits, coffee intake, wine, and beer consumption were major lifestyle determinants of tHcy. Changes in these lifestyle factors may reduce tHcy concentrations, thereby lowering cardiovascular risk in the general population.