Search resultsEffects of Alcohol on the Major Steps of Reverse Cholesterol Transport
Title
Effects of Alcohol on the Major Steps of Reverse Cholesterol Transport
Publication type
Journal Article
Year of Publication
2000
Authors
Journal
Biochemistry (Moscow)
Volume
65
Issue
11
Pagination
1310 - 1315
Date published
2000
ISBN
00062979 (ISSN)
Keywords
Adult, alcohol, Alcohol Drinking, article, Biological Transport, blood, Cholesterol, Cholesteryl ester transfer protein, drinking behavior, esterification, Ethanol, high density lipoprotein, human, Humans, Lecithin:cholesterol acyltransferase, male, metabolism, Middle Aged, phosphatidylcholine sterol acyltransferase, Phosphatidylcholine-Sterol O-Acyltransferase, Reverse cholesterol transport, transport at the cellular level
Abstract
The effects of moderate alcohol consumption on the capacity of blood sera to promote acceptance of cholesterol (C) from Fu5AH hepatoma cells, esterification of delivered free C, and transfer of produced cholesteryl esters to apolipoprotein (apo) B-containing lipoproteins have been studied. Twenty male subjects with relatively high (≥50 mg/dl, n = 10) and low (<50 mg/dl, n = 10) high density lipoprotein (HDL) C levels consumed for eight weeks red grape wine (0.3 g ethanol/kg body mass per day). Alcohol consumption reduced total C and low density lipoprotein C levels in both groups of subjects. Low HDL C subjects showed an increase in HDL C, apo AI, apo AII, and lipoprotein (Lp) AI particle levels after alcohol consumption. Alcohol did not affect free C efflux from the cells. However, after the following period of substitution of alcohol with an isocaloric amount of red grape juice, cellular C efflux markedly reduced. While lecithin:cholesterol acyltransferase (LCAT) activity increased during alcohol consumption only in subjects with low HDL C, high HDL C subjects showed a significant decrease in cholesteryl ester transfer protein (CETP) activity. At the same time, alcohol consumption reduced the endogenous C esterification rate and increased the transfer of endogenous cholesteryl esters to apo B-containing lipoproteins in both groups. Thus, alcohol consumption in moderate doses enhanced the anti-atherogenicity of the serum lipoprotein spectrum, supporting more effective C efflux from peripheral cells and transport of accepted C to apo B-containing lipoproteins. The effects of alcohol on the reverse cholesterol transport depend on the initial HDLC level.