Search resultsModerate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men
Title
Moderate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men
Publication type
Journal Article
Year of Publication
2012
Authors
Journal
British Journal of Nutrition
Volume
108
Issue
4
Pagination
620 - 627
Date published
2012
ISBN
00071145 (ISSN)
Keywords
adiponectin, Adult, alcohol consumption, Alcoholic Beverages, alpha 1 antitrypsin, alpha 1-Antitrypsin, article, blood cell, blood sampling, Cholesterol, HDL, Chronic Disease, controlled study, Cross-Over Studies, crossover procedure, Cytokines, down regulation, ferritin, Ferritins, gene control, gene expression, Gene Expression Profiling, gene expression regulation, genome, Genome-Wide Association Study, glucocorticoid receptor, Glucocorticoid signalling, high density lipoprotein cholesterol, human, human experiment, Humans, immune response, immunoglobulin enhancer binding protein, inflammation, Inflammation Mediators, interleukin 1 receptor blocking agent, interleukin 18, leukocyte, Leukocytes, lipid metabolism, male, Netherlands, NF-B, normal human, Oligonucleotide Array Sequence Analysis, open study, orange juice, peroxisome proliferator activated receptor alpha, randomized controlled trial, Receptors, Glucocorticoid, Risk, signal transduction, transcriptomics, upregulation, vodka, Young Adult
Abstract
Moderate alcohol consumption has various effects on immune and inflammatory processes, which could accumulatively modulate chronic disease risk. So far, no comprehensive, integrative profiling has been performed to investigate the effects of longer-term alcohol consumption. Therefore, we studied the effects of alcohol consumption on gene expression patterns using large-scale profiling of whole-genome transcriptomics in blood cells and on a number of proteins in blood. In a randomised, open-label, cross-over trial, twenty-four young, normal-weight men consumed 100 ml vodka (30 g alcohol) with 200 ml orange juice or only orange juice daily during dinner for 4 weeks. After each period, blood was sampled for measuring gene expression and selected proteins. Pathway analysis of 345 down-regulated and 455 up-regulated genes revealed effects of alcohol consumption on various signalling responses, immune processes and lipid metabolism. Among the signalling processes, the most prominently changed was glucocorticoid receptor signalling. A network on immune response showed a down-regulated NF-B gene expression together with increased plasma adiponectin and decreased pro-inflammatory IL-1 receptor antagonist and IL-18, and acute-phase proteins ferritin and α1-antitrypsin concentrations (all P < 0.05) after alcohol consumption. Furthermore, a network of gene expression changes related to lipid metabolism was observed, with a central role for PPARα which was supported by increased HDL-cholesterol and several apo concentrations (all P < 0.05) after alcohol consumption. In conclusion, an integrated approach of profiling both genes and proteins in blood showed that 4 weeks of moderate alcohol consumption altered immune responses and lipid metabolism.